Single-channel recordings of gramicidin at agarose-supported bilayer lipid membranes formed by the tip-dip and painting methods

Yuka Matsuno; Chie Osono; Ayumi Hirano; Masao Sugawara

doi:10.2116/analsci.20.1217

Single-channel recordings of gramicidin at agarose-supported bilayer lipid membranes formed by the tip-dip and painting methods

Yuka Matsuno, Chie Osono, Ayumi Hirano, Masao Sugawara

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of >1.0 × 10¹¹ Ω and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.

Original language	English
Pages (from-to)	1217-1221
Number of pages	5
Journal	analytical sciences
Volume	20
Issue number	8
DOIs	https://doi.org/10.2116/analsci.20.1217
Publication status	Published - 2004 Aug
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of >1.0 × 1011 Ω and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.",

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AU - Sugawara, Masao

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AB - Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of >1.0 × 1011 Ω and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.

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Single-channel recordings of gramicidin at agarose-supported bilayer lipid membranes formed by the tip-dip and painting methods

Abstract

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