Single-channel recordings of gramicidin at agarose-supported bilayer lipid membranes formed by the tip-dip and painting methods

Yuka Matsuno, Chie Osono, Ayumi Hirano, Masao Sugawara

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Agarose-supported BLMs were prepared by the tip-dip and painting methods, and single-channel recordings of gramicidin were examined for the development of an ion-channel sensor. The supported BLMs formed by the tip-dip method had an electric resistance of >1.0 × 10 11 Ω and a longer lifetime as compared with unsupported ones, which enabled single-channel recordings of gramicidin. The supported BLMs formed by the painting method also enabled single-channel recordings, but the lifetime was shorter than that of unsupported planar BLMs formed by the monolayer folding method.

Original languageEnglish
Pages (from-to)1217-1221
Number of pages5
Journalanalytical sciences
Volume20
Issue number8
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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