Single-channel analysis of a cloned human heart L-type Ca2+ channel α1 subunit and the effects of a cardiac β subunit

Minoru Wakamori, Gabor Mikala, Arnold Schwartz, Atsuko Yatani

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Macroscopic and single-channel properties of a cloned human heart L-type Ca2+ channel α1 subunit expressed in Xenopus oocytes were studied and the effects of a cardiac β subunit were evaluated. The α1 subunit expressed current with much slower activation and inactivation kinetics than native cells. The β subunit increased the current amplitude and accelerated both activation and inactivation rates. Single-channel analysis revealed that the β subunit increased the probability of channel opening and shifted the voltage-dependence to more negative potentials without affecting conductance or open time. The data suggest that the β subunit modulates macroscopic current by increasing the probability of channel opening and shifting the voltage-dependence of the channel.

Original languageEnglish
Pages (from-to)1170-1176
Number of pages7
JournalBiochemical and biophysical research communications
Volume196
Issue number3
DOIs
Publication statusPublished - 1993 Nov 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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