Single-cell level continuous observation of microorganism galvanotaxis using high-speed vision

Naoko Ogawa, Hiromasa Oku, Koichi Hashimoto, Masatoshi Ishikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

13 Citations (Scopus)

Abstract

A novel system for measurement of motile microorganism galvanotaxis using high-speed vision is presented. Our goal is to construct a smart microsystem composed of many controlled microorganisms called "Organized Bio-Modules" (OBM). The OEM system utilizes galvanotaxis (intrinsic reaction to electrical stimulus) of microorganisms for actuation. For evaluation of taxis, continuous observation of a moving single cell in a sufficiently large working area without fixation is needed. Using high-speed vision, we developed a system for continuous evaluation of galvanotaxis of freely swimming cells in a large area at the single-cell level. Experimental results demonstrate the continuous measurement of galvanotaxis of a Paramecium caudatum cell moving in a 3.5-mm-square area for 18 s with 1 μs precision.

Original languageEnglish
Title of host publication2004 2nd IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationMacro to Nano
Pages1331-1334
Number of pages4
Volume2
Publication statusPublished - 2004 Dec 1
Externally publishedYes
Event2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano - Arlington, VA, United States
Duration: 2004 Apr 152004 Apr 18

Other

Other2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
CountryUnited States
CityArlington, VA
Period04/4/1504/4/18

ASJC Scopus subject areas

  • Engineering(all)

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