Single blastomere removal from murine embryos is associated with activation of matrix metalloproteinases and janus kinase/signal transducers and activators of transcription pathways of placental inflammation

Brittany L.M. Sato, Atsushi Sugawara, Monika A. Ward, Abby C. Collier

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Single blastomere removal from cleavage-stage embryos, a common procedure used in conjunction with preimplantation genetic diagnosis (PGD), may affect reproductive outcomes.We hypothesized that negative pregnancy outcomes associated with PGD may be due to impairment of placental signaling pathways. The goal of this study was to determine the molecular mechanisms through which placental signaling is deregulated by blastomere removal. Four-cell stage murine embryos produced by in vitro fertilizationwere subjected to removal of a single blastomere (biopsied) or to the same manipulations without the blastomere removal (controls). Placental tissues from term (18.5 day) pregnancies obtained after embryo transfer were tested for levels of nitrosative species, interleukin 6, signal transducers and activators of transcription (STAT) 1 and 3, suppressors of cytokine signaling (SOCS) 1, 2 and 3 and matrix metalloproteinases (MMP) 1, 2, 3 and 9. Significant increases in nitrosative stress (P < 0.05), phosphorylative activation of STAT1 (P < 0.05) but not STAT3, lower levels of the inhibitors SOCS2 (P < 0.01) and SOCS3 (P < 0.001) and activation of MMP9 (P < 0.001) were observed in placentas derived from biopsied embryos, compared with controls. Such effects could contribute to greater levels of premature membrane rupture, incorrect parturition, preterm birth and intrauterine growth restriction associated with PGD. This work has determined signaling mechanisms that may be responsible for blastomere removal effects on placental function, with the potential to become targets for improving obstetric and neonatal outcomes in assisted reproduction.

Original languageEnglish
Pages (from-to)1247-1257
Number of pages11
JournalMolecular Human Reproduction
Volume20
Issue number12
DOIs
Publication statusPublished - 2014 Dec 1
Externally publishedYes

Keywords

  • Assisted reproduction technology
  • In vitro fertilization
  • Suppressors of cytokine signaling

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Single blastomere removal from murine embryos is associated with activation of matrix metalloproteinases and janus kinase/signal transducers and activators of transcription pathways of placental inflammation'. Together they form a unique fingerprint.

  • Cite this