Simultaneous quantification of seven prostanoids using liquid chromatography/tandem mass spectrometry: The effects of arachidonic acid on prostanoid production in mouse bone marrow-derived mast cells

Takanori Hishinuma, Kaori Suzuki, Masayoshi Saito, Hiroaki Yamaguchi, Naoto Suzuki, Yoshihisa Tomioka, Izumi Kaneko, Masao Ono, Junichi Goto

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We have developed a method for the simultaneous estimation of the levels of the prostanoids 6-keto prostaglandin (PG) F, PGB2, PGD2, PGE2, PGF, PGJ2, and thromboxane (TX) B2 in blood- or serum-containing medium using liquid chromatography-tandem mass spectrometry. These prostanoids and their deuterium derivatives, which were used as internal standards, were subjected to solid-phase extraction using Empore C18 HD disk cartridges and analyzed in the selected reaction-monitoring mode. A linear response curve starting at 10 pg of prostanoid/tube was observed for each prostanoid. The accuracy of the method was demonstrated with samples containing known amounts of the prostanoids. Furthermore, we used this method to analyze the prostanoids produced in mouse bone marrow-derived mast cells stimulated with arachidonic acid, which resulted in the production of PGD2, PGE2, PGF, and TXB2. The results suggest that this simultaneous quantification method is useful for the analysis of the production of biomedically important prostanoids.

Original languageEnglish
Pages (from-to)321-329
Number of pages9
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume76
Issue number6
DOIs
Publication statusPublished - 2007 Jun

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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