Simultaneous quantification of prostaglandins in human synovial cell-cultured medium using liquid chromatography/tandem mass spectrometry

M. Takabatake, T. Hishinuma, Naoto Suzuki, S. Chiba, Hiroki Tsukamoto, H. Nakamura, T. Saga, Y. Tomioka, A. Kurose, T. Sawai, M. Mizugaki

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

A liquid chromatographic-tandem mass spectrometric (LC/MS-MS) method was developed for the simultaneous quantification of prostaglandin (PG) E 2 , PGF , 6-keto-PGF and thromboxane (TX) B 2 . These eicosanoids and their deuterium derivatives, using as internal standards, were extracted by solid-phase extraction and analyzed using LC/MS-MS in the selected reaction-monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng for each eicosanoid was demonstrated. The accuracy of added eicosanoids ranged from 94.1 to 106.6% and coefficients of variation ranged from 0.62 to 7.8%. Furthermore, we applied this method for the determination of eicosanoids in the human synovial cell-cultured medium, stimulated by lipopolysaccharide (LPS). LPS produced each eicosanoid and they increased in a time-dependent manner. The production levels after 24 h stimulation were 6-keto-PGF >PGE 2 >TXB 2 ≫PGF . This simultaneous quantification method is so useful to clarify the function of synovial cells in rheumatoid arthritis (RA).

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume67
Issue number1
DOIs
Publication statusPublished - 2002 Jul 1

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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