A liquid chromatographic-tandem mass spectrometric (LC/MS-MS) method was developed for the simultaneous quantification of prostaglandin (PG) E 2 , PGF 2α , 6-keto-PGF 1α and thromboxane (TX) B 2 . These eicosanoids and their deuterium derivatives, using as internal standards, were extracted by solid-phase extraction and analyzed using LC/MS-MS in the selected reaction-monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng for each eicosanoid was demonstrated. The accuracy of added eicosanoids ranged from 94.1 to 106.6% and coefficients of variation ranged from 0.62 to 7.8%. Furthermore, we applied this method for the determination of eicosanoids in the human synovial cell-cultured medium, stimulated by lipopolysaccharide (LPS). LPS produced each eicosanoid and they increased in a time-dependent manner. The production levels after 24 h stimulation were 6-keto-PGF 1α >PGE 2 >TXB 2 ≫PGF 2α . This simultaneous quantification method is so useful to clarify the function of synovial cells in rheumatoid arthritis (RA).
|Number of pages||6|
|Journal||Prostaglandins Leukotrienes and Essential Fatty Acids|
|Publication status||Published - 2002 Jul 1|
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology