Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin

Akihiko Ishijima, Hiroaki Kojima, Takashi Funatsu, Makio Tokunaga, Hideo Higuchi, Hiroto Tanaka, Toshio Yanagida

Research output: Contribution to journalArticlepeer-review

416 Citations (Scopus)

Abstract

We have developed a technique that allows mechanical and ligand-binding events in a single myosin molecule to be monitored simultaneously. We describe how steps in the ATPase reaction are temporally related to mechanical events at the single molecule level. The results show that the force generation does not always coincide with the release of bound nucleotide, presumably ADP. Instead the myosin head produces force several hundreds of milliseconds after bound nucleotide is released. This finding does not support the widely accepted view that force generation is directly coupled to the release of bound ligands. It suggests that myosin has a hysteresis or memory state, which stores chemical energy from ATP hydrolysis.

Original languageEnglish
Pages (from-to)161-171
Number of pages11
JournalCell
Volume92
Issue number2
DOIs
Publication statusPublished - 1998 Jan 23

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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