Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

Shizuko Hozumi; Kana Ikezawa; Atushi Shoji; Ayumi Hirano-Iwata; Tim Bliss; Masao Sugawara

doi:10.1016/j.bios.2010.11.052

Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

Shizuko Hozumi, Kana Ikezawa, Atushi Shoji, Ayumi Hirano-Iwata, Tim Bliss, Masao Sugawara

Advanced Institute for Materials Research (AIMR)

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Simultaneous monitoring of amperometric currents at a glass capillary sensor based on recombinant GluOx and field excitatory postsynaptic potentials (fEPSPs) were performed in region CA1 of mouse hippocampal slices. A transient increase in the glutamate current relative to the basal one at control stimulation (0.052. Hz) was evoked by stimulation at 2. Hz for 2. min. The magnitude of the glutamate current was dependent on the intensity (current) of a 2. Hz stimulus and reflected the slope of the fEPSP. The in situ calibration of the l-glutamate sensor revealed that the extracellular concentration of l-glutamate released by 2. Hz stimulation before tetanus is in the range from 0.8 to 2.2. μM and it is enhanced after tetanic stimulation. The l-glutamate level at a test stimulus (0.052. Hz) was estimated to be 32. nM. The recombinant GluOx-based sensor exhibited weak responses to glutamine above 300. μM and l-aspartic acid above 200. μM. The potential use of a glass capillary sensor in combination with fEPSP measurements for electrophysiological study is discussed.

Original language	English
Pages (from-to)	2975-2980
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	6
DOIs	https://doi.org/10.1016/j.bios.2010.11.052
Publication status	Published - 2011 Feb 15

Keywords

CA1
Field excitatory postsynaptic potentials
Glutamate currents
L-Glutamate
Mouse hippocampal slice

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2010.11.052

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title = "Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices",

abstract = "Simultaneous monitoring of amperometric currents at a glass capillary sensor based on recombinant GluOx and field excitatory postsynaptic potentials (fEPSPs) were performed in region CA1 of mouse hippocampal slices. A transient increase in the glutamate current relative to the basal one at control stimulation (0.052. Hz) was evoked by stimulation at 2. Hz for 2. min. The magnitude of the glutamate current was dependent on the intensity (current) of a 2. Hz stimulus and reflected the slope of the fEPSP. The in situ calibration of the l-glutamate sensor revealed that the extracellular concentration of l-glutamate released by 2. Hz stimulation before tetanus is in the range from 0.8 to 2.2. μM and it is enhanced after tetanic stimulation. The l-glutamate level at a test stimulus (0.052. Hz) was estimated to be 32. nM. The recombinant GluOx-based sensor exhibited weak responses to glutamine above 300. μM and l-aspartic acid above 200. μM. The potential use of a glass capillary sensor in combination with fEPSP measurements for electrophysiological study is discussed.",

keywords = "CA1, Field excitatory postsynaptic potentials, Glutamate currents, L-Glutamate, Mouse hippocampal slice",

author = "Shizuko Hozumi and Kana Ikezawa and Atushi Shoji and Ayumi Hirano-Iwata and Tim Bliss and Masao Sugawara",

note = "Funding Information: This work was financially supported by Grant-in-Aid for Scientific and Research (B) (no. 21350045 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan . Financial support from a research grant from the College of Humanities and Sciences at Nihon University is also acknowledged. ",

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AU - Ikezawa, Kana

AU - Shoji, Atushi

AU - Hirano-Iwata, Ayumi

AU - Bliss, Tim

AU - Sugawara, Masao

N1 - Funding Information: This work was financially supported by Grant-in-Aid for Scientific and Research (B) (no. 21350045 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan . Financial support from a research grant from the College of Humanities and Sciences at Nihon University is also acknowledged.

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Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

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