Simultaneous isolation and concentration of target solute from multicomponent mixture using continuous rotating annular chromatograph with double-stage partial recycling of effluent

Takuya Fukumura, Masaki Kubo, Toshikuni Yonemoto

Research output: Contribution to journalArticle

Abstract

We propose a novel technique for the simultaneous isolation and concentration of a target solute from a multicomponent mixture using a continuous rotating annular chromatograph with double-stage partial recycling of the effluent. In this technique, the target solute is first completely isolated from the multicomponent mixture by a partial recycling method in which the effluents of incomplete separation are recycled to the inlet adjacent to that of the raw feed mixture. Next, the isolated solute is re-recycled to the inlet of the region unused for isolation. In order to concentrate the isolated solute, a second eluent removing the solute from the chromatographic packing to the external liquid phase and making the elution band narrower is supplied to the inlet at a larger angle than that of the re-recycled solute. The technique was applied to the simultaneous isolation and concentration of valine from a ternary mixture of amino acids, glutamic acid, valine and leucine. The numerical calculation based on a mass transport model was used to determine the optimum arrangement of the recycling channels and their corresponding nozzles for isolation and concentration of the target solute. The double-stage partial recycling method allowed the target solute, valine, to be completely isolated and concentrated from the initial concentration of 0.1 mol/m3 to 0.233 mol/m3.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalJournal of Chemical Engineering of Japan
Volume37
Issue number2
DOIs
Publication statusPublished - 2004 Feb 1

Keywords

  • Annular chromatography
  • Concentration
  • Isolation
  • Partial recycling
  • Step elution

ASJC Scopus subject areas

  • Chemical Engineering(all)

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