Simultaneous in situ detection of alkaline phosphatase activity and polyphosphate in arbuscules within arbuscular mycorrhizal roots

Rintaro Funamoto, Katsuharu Saito, Hiroshi Oyaizu, Masanori Saito, Toshihiro Aono

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Inorganic phosphate (Pi) metabolism in arbuscules of arbuscular mycorrhizal (AM) fungi is not well understood, although recent research has revealed that host plants absorb Pi around arbuscules with mycorrhiza-specific transporters. Therefore, we analysed the localisation of polyphosphate (polyP) and alkaline phosphatase (ALP) activity in arbuscules, which could be indicators of Pi metabolism. We developed a dual-labelling method for polyP and ALP activity, i.e. first labelling with fluorescent probes 4′,6-diamidino-2-phenyl- indole dihydrochloride (DAPI) and then labelling with enzyme-labelled fluorescence (ELF97). The dual-labelling method made it possible to observe polyP and ALP activity signals simultaneously in mycorrhizal roots. The dual-labelling method revealed that ALP activity was mainly observed in mature arbuscules where polyP was rarely observed. The expression of the AM fungal ALP gene was suppressed in the knockdown plants of an AM-inducible Pi-transporter, and there was much polyP in arbuscules that showed low ALP activity. These topological observations suggest that there may be some relationships between polyP metabolism and ALP activity in arbuscules, and that these are, in part, controlled by Pi uptake by plants via the AM-inducible Pi-transporter.

Original languageEnglish
Pages (from-to)803-810
Number of pages8
JournalFunctional Plant Biology
Volume34
Issue number9
DOIs
Publication statusPublished - 2007 Sep 6

Keywords

  • Dual-labelling method
  • Fluorescent probe
  • Glomus intraradices
  • Lotus japonicus
  • RNA interference

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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