Simultaneous imaging of an enantiomer pair by electron paramagnetic resonance using isotopic nitrogen labeling

Yusuke Miyake, Xiaolei Wang, Mitsuo Amasaka, Kaori Itto, Shu Xu, Hirokazu Arimoto, Hirotada Fujii, Hiroshi Hirata

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    This Article describes the simultaneous imaging of chiral nitroxyl radicals using electron paramagnetic resonance (EPR). Chiral nitroxyl radicals could be simultaneously visualized with the labeling of isotopic nitrogen. Chiral nitroxyl radicals, hydroxylmethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl, were visualized using the method of simultaneous EPR imaging, which refers to the visualization of two kinds of molecules with unpaired electrons in a single image scan. EPR spectra of a racemic mixture of chiral nitroxyl radicals and those of the respective R and S configurations confirmed labeling by isotopic nitrogen. 1H nuclear magnetic resonance (NMR) imaging and simultaneous imaging of solutions of chiral nitroxyl radicals were performed. The advantages and limitations of simultaneous imaging using EPR are also discussed. Simultaneous imaging with chiral-labeled nitroxyl radicals is a new application of EPR imaging and may be useful for biological studies involving biologically active chiral molecules.

    Original languageEnglish
    Pages (from-to)985-990
    Number of pages6
    JournalAnalytical Chemistry
    Volume85
    Issue number2
    DOIs
    Publication statusPublished - 2013 Jan 15

    ASJC Scopus subject areas

    • Analytical Chemistry

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