Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS

Kiyotaka Usui; Takanori Hishinuma; Hiroaki Yamaguchi; Toshihide Saga; Toshihiro Wagatsuma; Kunihiko Hoshi; Naoki Tachiiri; Kuniaki Miura; Junichi Goto

doi:10.1016/j.legalmed.2006.02.001

Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS

Kiyotaka Usui, Takanori Hishinuma, Hiroaki Yamaguchi, Toshihide Saga, Toshihiro Wagatsuma, Kunihiko Hoshi, Naoki Tachiiri, Kuniaki Miura, Junichi Goto

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC-ESI-MS conditions, these compounds were almost fully separated within 6.5 min. Linear responses over the concentration range 0.25-50.0 ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.

Original language	English
Pages (from-to)	166-171
Number of pages	6
Journal	Legal Medicine
Volume	8
Issue number	3
DOIs	https://doi.org/10.1016/j.legalmed.2006.02.001
Publication status	Published - 2006 May

Keywords

Determination
Forensic analysis
LC-ESI-MS
Pancuronium
Toxicology
Vecuronium

ASJC Scopus subject areas

Pathology and Forensic Medicine
Issues, ethics and legal aspects

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Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS. / Usui, Kiyotaka; Hishinuma, Takanori; Yamaguchi, Hiroaki; Saga, Toshihide; Wagatsuma, Toshihiro; Hoshi, Kunihiko; Tachiiri, Naoki; Miura, Kuniaki; Goto, Junichi.

In: Legal Medicine, Vol. 8, No. 3, 05.2006, p. 166-171.

Research output: Contribution to journal › Article › peer-review

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abstract = "A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC-ESI-MS conditions, these compounds were almost fully separated within 6.5 min. Linear responses over the concentration range 0.25-50.0 ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.",

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AU - Hishinuma, Takanori

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AU - Saga, Toshihide

AU - Wagatsuma, Toshihiro

AU - Hoshi, Kunihiko

AU - Tachiiri, Naoki

AU - Miura, Kuniaki

AU - Goto, Junichi

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KW - Toxicology

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