TY - JOUR
T1 - Simultaneous determination of pancuronium, vecuronium and their related compounds using LC-ESI-MS
AU - Usui, Kiyotaka
AU - Hishinuma, Takanori
AU - Yamaguchi, Hiroaki
AU - Saga, Toshihide
AU - Wagatsuma, Toshihiro
AU - Hoshi, Kunihiko
AU - Tachiiri, Naoki
AU - Miura, Kuniaki
AU - Goto, Junichi
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2006/5
Y1 - 2006/5
N2 - A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC-ESI-MS conditions, these compounds were almost fully separated within 6.5 min. Linear responses over the concentration range 0.25-50.0 ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.
AB - A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC-ESI-MS conditions, these compounds were almost fully separated within 6.5 min. Linear responses over the concentration range 0.25-50.0 ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.
KW - Determination
KW - Forensic analysis
KW - LC-ESI-MS
KW - Pancuronium
KW - Toxicology
KW - Vecuronium
UR - http://www.scopus.com/inward/record.url?scp=33646776079&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646776079&partnerID=8YFLogxK
U2 - 10.1016/j.legalmed.2006.02.001
DO - 10.1016/j.legalmed.2006.02.001
M3 - Article
C2 - 16621652
AN - SCOPUS:33646776079
VL - 8
SP - 166
EP - 171
JO - Legal Medicine
JF - Legal Medicine
SN - 1344-6223
IS - 3
ER -