Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme-modified Large-scale Integration (LSI)-based Amperometric Electrochemical Device

Hiroya Abe, Tomoki Iwama, Hiroshi Yabu, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme-modified large-scale integration (LSI)-based amperometric devices, referred to “Bio-LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer-modified electrodes. The Os-HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme-modified Bio-LSI.

Original languageEnglish
Pages (from-to)2841-2846
Number of pages6
JournalElectroanalysis
Volume30
Issue number12
DOIs
Publication statusPublished - 2018 Dec

Keywords

  • Biosensor
  • Electrochemical imaging
  • Neurotransmitters

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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