Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme-modified Large-scale Integration (LSI)-based Amperometric Electrochemical Device

Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme-modified large-scale integration (LSI)-based amperometric devices, referred to “Bio-LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer-modified electrodes. The Os-HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme-modified Bio-LSI.