Abstract
Microgravity has advantages for the cultivation of tissues with high fidelity; however, tissue formation requires cellular recognition and adhesion. We tested the hypothesis that simulated microgravity does not affect cell adhesion. Human colorectal carcinoma cells were cultured in the NASA Rotating Wall Vessel (RWV) under low shear stress with randomization of the gravity vector that simulates microgravity. After 6 - 7 days, cells were assayed for binding to various substrates and compared to cells grown in standard tissue culture flasks and static suspension cultures. The RWV cultures bound as well to basement membrane proteins and to CEA, an intercellular adhesion molecule, as control cultures did. Thus, microgravity does not alter epithelial cell adhesion and may be useful for tissue engineering.
Original language | English |
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Pages (from-to) | 71-76 |
Number of pages | 6 |
Journal | Advances in Space Research |
Volume | 14 |
Issue number | 8 |
DOIs | |
Publication status | Published - 1994 Aug |
Externally published | Yes |
ASJC Scopus subject areas
- Aerospace Engineering
- Astronomy and Astrophysics
- Geophysics
- Atmospheric Science
- Space and Planetary Science
- Earth and Planetary Sciences(all)