Simulated microgravity does not alter epithelial cell adhesion to matrix and other molecules

J. M. Jessup, K. Brown, S. Ishii, R. Ford, T. J. Goodwin, G. Spaulding

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Microgravity has advantages for the cultivation of tissues with high fidelity; however, tissue formation requires cellular recognition and adhesion. We tested the hypothesis that simulated microgravity does not affect cell adhesion. Human colorectal carcinoma cells were cultured in the NASA Rotating Wall Vessel (RWV) under low shear stress with randomization of the gravity vector that simulates microgravity. After 6 - 7 days, cells were assayed for binding to various substrates and compared to cells grown in standard tissue culture flasks and static suspension cultures. The RWV cultures bound as well to basement membrane proteins and to CEA, an intercellular adhesion molecule, as control cultures did. Thus, microgravity does not alter epithelial cell adhesion and may be useful for tissue engineering.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalAdvances in Space Research
Volume14
Issue number8
DOIs
Publication statusPublished - 1994 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Aerospace Engineering
  • Astronomy and Astrophysics
  • Geophysics
  • Atmospheric Science
  • Space and Planetary Science
  • Earth and Planetary Sciences(all)

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