Simplified enzymatic synthesis and biodistribution of 11C-S-adenosyl-l-methionine

Kiichi Ishiwata; Tatsuo Ido; Hiromi Sato; Ren Iwata; Koichiro Kawashima; Kazuhiko Yanai; Shoichi Watanuki; Hitoshi Ohtomo; Kyuya Kogure

doi:10.1007/BF00261008

Simplified enzymatic synthesis and biodistribution of ¹¹C-S-adenosyl-l-methionine

Kiichi Ishiwata, Tatsuo Ido, Hiromi Sato, Ren Iwata, Koichiro Kawashima, Kazuhiko Yanai, Shoichi Watanuki, Hitoshi Ohtomo, Kyuya Kogure

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

¹¹C-S-Adenosyl-l-methionine (¹¹C-SAM) was synthesized enzymatically from ¹¹C-l-methionine using ratliver extract [40%-50% saturated (NH₄)₂SO₄ fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of ¹¹C-SAM was found in the kidneys. ¹¹C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of ¹¹C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the ¹¹C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of ¹¹C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of ¹¹-SAM clearly visualized using positronemission tomography.

Original language	English
Pages (from-to)	449-452
Number of pages	4
Journal	European Journal of Nuclear Medicine
Volume	11
Issue number	11
DOIs	https://doi.org/10.1007/BF00261008
Publication status	Published - 1986 Mar 1

Keywords

C-S-Adenosyl-l-methionine
Kidney imaging
Positron

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Access to Document

10.1007/BF00261008

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Ishiwata, K., Ido, T., Sato, H., Iwata, R., Kawashima, K., Yanai, K., Watanuki, S., Ohtomo, H., & Kogure, K. (1986). Simplified enzymatic synthesis and biodistribution of ¹¹C-S-adenosyl-l-methionine. European Journal of Nuclear Medicine, 11(11), 449-452. https://doi.org/10.1007/BF00261008

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title = "Simplified enzymatic synthesis and biodistribution of 11C-S-adenosyl-l-methionine",

abstract = "11C-S-Adenosyl-l-methionine (11C-SAM) was synthesized enzymatically from 11C-l-methionine using ratliver extract [40%-50% saturated (NH4)2SO4 fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of 11C-SAM was found in the kidneys. 11C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of 11C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the 11C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of 11C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of 11-SAM clearly visualized using positronemission tomography.",

keywords = "C-S-Adenosyl-l-methionine, Kidney imaging, Positron",

author = "Kiichi Ishiwata and Tatsuo Ido and Hiromi Sato and Ren Iwata and Koichiro Kawashima and Kazuhiko Yanai and Shoichi Watanuki and Hitoshi Ohtomo and Kyuya Kogure",

year = "1986",

month = mar,

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doi = "10.1007/BF00261008",

language = "English",

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T1 - Simplified enzymatic synthesis and biodistribution of 11C-S-adenosyl-l-methionine

AU - Ishiwata, Kiichi

AU - Ido, Tatsuo

AU - Sato, Hiromi

AU - Iwata, Ren

AU - Kawashima, Koichiro

AU - Yanai, Kazuhiko

AU - Watanuki, Shoichi

AU - Ohtomo, Hitoshi

AU - Kogure, Kyuya

PY - 1986/3/1

Y1 - 1986/3/1

N2 - 11C-S-Adenosyl-l-methionine (11C-SAM) was synthesized enzymatically from 11C-l-methionine using ratliver extract [40%-50% saturated (NH4)2SO4 fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of 11C-SAM was found in the kidneys. 11C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of 11C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the 11C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of 11C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of 11-SAM clearly visualized using positronemission tomography.

AB - 11C-S-Adenosyl-l-methionine (11C-SAM) was synthesized enzymatically from 11C-l-methionine using ratliver extract [40%-50% saturated (NH4)2SO4 fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of 11C-SAM was found in the kidneys. 11C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of 11C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the 11C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of 11C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of 11-SAM clearly visualized using positronemission tomography.

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