TY - JOUR
T1 - Simplified enzymatic synthesis and biodistribution of 11C-S-adenosyl-l-methionine
AU - Ishiwata, Kiichi
AU - Ido, Tatsuo
AU - Sato, Hiromi
AU - Iwata, Ren
AU - Kawashima, Koichiro
AU - Yanai, Kazuhiko
AU - Watanuki, Shoichi
AU - Ohtomo, Hitoshi
AU - Kogure, Kyuya
PY - 1986/3
Y1 - 1986/3
N2 - 11C-S-Adenosyl-l-methionine (11C-SAM) was synthesized enzymatically from 11C-l-methionine using ratliver extract [40%-50% saturated (NH4)2SO4 fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of 11C-SAM was found in the kidneys. 11C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of 11C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the 11C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of 11C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of 11-SAM clearly visualized using positronemission tomography.
AB - 11C-S-Adenosyl-l-methionine (11C-SAM) was synthesized enzymatically from 11C-l-methionine using ratliver extract [40%-50% saturated (NH4)2SO4 fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of 11C-SAM was found in the kidneys. 11C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of 11C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the 11C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of 11C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of 11-SAM clearly visualized using positronemission tomography.
KW - C-S-Adenosyl-l-methionine
KW - Kidney imaging
KW - Positron
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U2 - 10.1007/BF00261008
DO - 10.1007/BF00261008
M3 - Article
C2 - 3486766
AN - SCOPUS:0022654980
VL - 11
SP - 449
EP - 452
JO - European Journal of Nuclear Medicine and Molecular Imaging
JF - European Journal of Nuclear Medicine and Molecular Imaging
SN - 1619-7070
IS - 11
ER -