Simplified enzymatic synthesis and biodistribution of 11C-S-adenosyl-l-methionine

Kiichi Ishiwata, Tatsuo Ido, Hiromi Sato, Ren Iwata, Koichiro Kawashima, Kazuhiko Yanai, Shoichi Watanuki, Hitoshi Ohtomo, Kyuya Kogure

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

11C-S-Adenosyl-l-methionine (11C-SAM) was synthesized enzymatically from 11C-l-methionine using ratliver extract [40%-50% saturated (NH4)2SO4 fraction] as the enzyme source. In biodistribution studies in rats, the highest uptake of 11C-SAM was found in the kidneys. 11C-SAM was also accumulated in the small intestine, pancreas, adrenal gland, liver, and spleen. The uptake of 11C-SAM in the brain increased with time, but remained low. At 30 min after injection, about 50%-60% of the 11C radioactivity was present in the acid-insoluble fraction of the kidneys and liver. When a high loading dose of 11C-SAM was administered, the kidney uptake was enhanced, but the proportion of the radioactivity present in the acid-insoluble fraction was lower. In a study of one rabbit, the kidney uptake was of 11-SAM clearly visualized using positronemission tomography.

Original languageEnglish
Pages (from-to)449-452
Number of pages4
JournalEuropean Journal of Nuclear Medicine
Volume11
Issue number11
DOIs
Publication statusPublished - 1986 Mar 1

Keywords

  • C-S-Adenosyl-l-methionine
  • Kidney imaging
  • Positron

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Simplified enzymatic synthesis and biodistribution of <sup>11</sup>C-S-adenosyl-l-methionine'. Together they form a unique fingerprint.

  • Cite this

    Ishiwata, K., Ido, T., Sato, H., Iwata, R., Kawashima, K., Yanai, K., Watanuki, S., Ohtomo, H., & Kogure, K. (1986). Simplified enzymatic synthesis and biodistribution of 11C-S-adenosyl-l-methionine. European Journal of Nuclear Medicine, 11(11), 449-452. https://doi.org/10.1007/BF00261008