Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry

Takanori Hishinuma, Kaori Suzuki, Hiroaki Yamaguchi, Hatsushi Yamagishi, Tomoyuki Koike, Shuichi Ohara, Toru Shimosegawa, Nariyasu Mano, Junichi Goto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 μL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1 mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 μg/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.

Original languageEnglish
Pages (from-to)38-45
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume870
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1

Keywords

  • Determination
  • Human
  • LC/MS/MS
  • Lansoprazole
  • Rabeprazole
  • Serum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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