Simple multiplex PCR assay for identification of Beijing family Mycobacterium tuberculosis isolates with a lineage-specific mutation in Rv0679c

Chie Nakajima, Aki Tamaru, Zeaur Rahim, Ajay Poudel, Bhagwan Maharjan, Khin Saw Aye, Hong Ling, Toshio Hattori, Tomotada Iwamoto, Yukari Fukushima, Haruka Suzuki, Yasuhiko Suzuki, Takashi Matsuba

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The Beijing genotype of Mycobacterium tuberculosis is known to be a worldwide epidemic clade. It is suggested to be a possibly resistant clone against BCG vaccination and is also suggested to be highly pathogenic and prone to becoming drug resistant. Thus, monitoring the prevalence of this lineage seems to be important for the proper control of tuberculosis. The Rv0679c protein of M. tuberculosis has been predicted to be one of the outer membrane proteins and is suggested to contribute to host cell invasion. Here, we conducted a sequence analysis of the Rv0679c gene using clinical isolates and found that a single nucleotide polymorphism, C to G at position 426, can be observed only in the isolates that are identified as members of the Beijing genotype family. Here, we developed a simple multiplex PCR assay to detect this point mutation and applied it to 619 clinical isolates. The method successfully distinguished Beijing lineage clones from non-Beijing strains with 100% accuracy. This simple, quick, and cost-effective multiplex PCR assay can be used for a survey or for monitoring the prevalence of Beijing genotype M. tuberculosis strains.

Original languageEnglish
Pages (from-to)2025-2032
Number of pages8
JournalJournal of Clinical Microbiology
Volume51
Issue number7
DOIs
Publication statusPublished - 2013 Jul

ASJC Scopus subject areas

  • Microbiology (medical)

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