Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane

Shizuya Kabuto; Koji Muramoto

doi:10.1271/bbb.62.188

Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane

Shizuya Kabuto, Koji Muramoto

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4' -azobenzene sulfonyl hydrazide. The glycoproteins could be visualized as red-colored spots or bands in the range of 0.16-0.31 μg.

Original language	English
Pages (from-to)	188-189
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	62
Issue number	1
DOIs	https://doi.org/10.1271/bbb.62.188
Publication status	Published - 1998

Keywords

PVDF membrane
blotting
glycoprotein
hydrazide
sugar

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4' -azobenzene sulfonyl hydrazide. The glycoproteins could be visualized as red-colored spots or bands in the range of 0.16-0.31 μg.",

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author = "Shizuya Kabuto and Koji Muramoto",

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language = "English",

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T1 - Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane

AU - Kabuto, Shizuya

AU - Muramoto, Koji

PY - 1998

Y1 - 1998

N2 - A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4' -azobenzene sulfonyl hydrazide. The glycoproteins could be visualized as red-colored spots or bands in the range of 0.16-0.31 μg.

AB - A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4' -azobenzene sulfonyl hydrazide. The glycoproteins could be visualized as red-colored spots or bands in the range of 0.16-0.31 μg.

KW - PVDF membrane

KW - blotting

KW - glycoprotein

KW - hydrazide

KW - sugar

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Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane

Abstract

Keywords

ASJC Scopus subject areas

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