Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane

Shizuya Kabuto, Koji Muramoto

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4' -azobenzene sulfonyl hydrazide. The glycoproteins could be visualized as red-colored spots or bands in the range of 0.16-0.31 μg.

    Original languageEnglish
    Pages (from-to)188-189
    Number of pages2
    JournalBioscience, Biotechnology and Biochemistry
    Volume62
    Issue number1
    DOIs
    Publication statusPublished - 1998

    Keywords

    • PVDF membrane
    • blotting
    • glycoprotein
    • hydrazide
    • sugar

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

    Fingerprint Dive into the research topics of 'Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane'. Together they form a unique fingerprint.

    Cite this