Simple and sensitive quantitation method for mevalonic acid in plasma using gas chromatography/mass spectrometry

Yasushi Ishihama, Nariyasu Mano, Yoshiya Oda, Hironobu Hiyoshi, Hironori Ikuta, Yutaka Yoshida

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The combination of gas chromatography and mass spectrometry using a polar capillary column and isobutane chemical ionization made it possible to determine mevalonic acid (MVA) as the lactone at subnanogram levels using 1 mL of plasma. The pretreatment procedure consisted of only three steps, namely lactonization, washing with chloroform and liquid/liquid extraction. This simple, rapid and sensitive method, having good precision and accuracy, is useful for evaluating the change of plasma MVA, which is well correlated with whole‐body cholesterol biosynthesis.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume8
Issue number5
DOIs
Publication statusPublished - 1994 May
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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