Simple and rapid detection of HLA-DRB polymorphism from forensic samples

T. Kimura, M. Nata, M. Hashiyada, M. Funayama, K. Sagisaka

Research output: Contribution to journalArticlepeer-review

Abstract

Simple and rapid detection of HLA-DRB polymorphism has been performed using AMPLICOR HLA-DRB Typing Kit. We tried to apply this kit to various forensic samples. When DNA was extracted from the forensic samples using conventional phenol-chloroform method, addition of 7.5mM MgCl2 was required to PCR amplification. HLA-DRB types were detected from DNA more than 0.1ng by PCR amplification. Typing of unrelated 50 Japanese showed 38 different patterns, of which 30 patterns occurred once in the group. A total of 16 serotypes were deduced from the HLA-DRB DNA types. Out of them, high frequency serotypes were DR4 (24%), DR9 (18%) and DR15/16 (14%). This kit was very useful in forensic cases such as rape and in paternity cases. When we tried to detect HLA-DRB types from a single hair shaft of 3 cm in length, we were successful in detection from only one of five persons.

Original languageEnglish
Pages (from-to)238-244
Number of pages7
JournalJapanese Journal of Legal Medicine
Volume52
Issue number4
Publication statusPublished - 1998 Dec 1
Externally publishedYes

Keywords

  • Amplicor
  • Forensic sample
  • HLA-DRB
  • Serotype

ASJC Scopus subject areas

  • Health Policy
  • Law

Fingerprint Dive into the research topics of 'Simple and rapid detection of HLA-DRB polymorphism from forensic samples'. Together they form a unique fingerprint.

Cite this