Abstract
Simple and rapid detection of HLA-DRB polymorphism has been performed using AMPLICOR HLA-DRB Typing Kit. We tried to apply this kit to various forensic samples. When DNA was extracted from the forensic samples using conventional phenol-chloroform method, addition of 7.5mM MgCl2 was required to PCR amplification. HLA-DRB types were detected from DNA more than 0.1ng by PCR amplification. Typing of unrelated 50 Japanese showed 38 different patterns, of which 30 patterns occurred once in the group. A total of 16 serotypes were deduced from the HLA-DRB DNA types. Out of them, high frequency serotypes were DR4 (24%), DR9 (18%) and DR15/16 (14%). This kit was very useful in forensic cases such as rape and in paternity cases. When we tried to detect HLA-DRB types from a single hair shaft of 3 cm in length, we were successful in detection from only one of five persons.
Original language | English |
---|---|
Pages (from-to) | 238-244 |
Number of pages | 7 |
Journal | Japanese Journal of Legal Medicine |
Volume | 52 |
Issue number | 4 |
Publication status | Published - 1998 Dec 1 |
Externally published | Yes |
Keywords
- Amplicor
- Forensic sample
- HLA-DRB
- Serotype
ASJC Scopus subject areas
- Health Policy
- Law