Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike

Nobuhiro Fujii; Kouichi Kimura; Noriko Yokosawa; Keiji Oguma; Teruo Yashiki; Touru Ohyama; T. Takeshi; Emiko Isogai; Hiroshi Isogai

doi:10.1111/j.1348-0421.1993.tb03227.x

Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike

Nobuhiro Fujii, Kouichi Kimura, Noriko Yokosawa, Keiji Oguma, Teruo Yashiki, Touru Ohyama, T. Takeshi, Emiko Isogai, Hiroshi Isogai

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb Hindlll fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.

Original language	English
Pages (from-to)	774-777
Number of pages	4
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	37
Issue number	5
DOIs	https://doi.org/10.1111/j.1348-0421.1993.tb03227.x
Publication status	Published - 1993
Externally published	Yes

Keywords

Botulinum toxin
Clostridium butyricum
Gene cloning
Neurotoxin
Progenitor toxin

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1993.tb03227.x

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Fujii, N., Kimura, K., Yokosawa, N., Oguma, K., Yashiki, T., Ohyama, T., Takeshi, T., Isogai, E., & Isogai, H. (1993). Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike. MICROBIOLOGY and IMMUNOLOGY, 37(5), 774-777. https://doi.org/10.1111/j.1348-0421.1993.tb03227.x

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 37, No. 5, 1993, p. 774-777.

Research output: Contribution to journal › Article › peer-review

Fujii, N, Kimura, K, Yokosawa, N, Oguma, K, Yashiki, T, Ohyama, T, Takeshi, T, Isogai, E & Isogai, H 1993, 'Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike', MICROBIOLOGY and IMMUNOLOGY, vol. 37, no. 5, pp. 774-777. https://doi.org/10.1111/j.1348-0421.1993.tb03227.x

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abstract = "The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb Hindlll fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.",

keywords = "Botulinum toxin, Clostridium butyricum, Gene cloning, Neurotoxin, Progenitor toxin",

author = "Nobuhiro Fujii and Kouichi Kimura and Noriko Yokosawa and Keiji Oguma and Teruo Yashiki and Touru Ohyama and T. Takeshi and Emiko Isogai and Hiroshi Isogai",

year = "1993",

doi = "10.1111/j.1348-0421.1993.tb03227.x",

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T1 - Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike

AU - Fujii, Nobuhiro

AU - Kimura, Kouichi

AU - Yokosawa, Noriko

AU - Oguma, Keiji

AU - Yashiki, Teruo

AU - Ohyama, Touru

AU - Takeshi, T.

AU - Isogai, Emiko

AU - Isogai, Hiroshi

PY - 1993

Y1 - 1993

N2 - The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb Hindlll fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.

AB - The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb Hindlll fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.

KW - Botulinum toxin

KW - Clostridium butyricum

KW - Gene cloning

KW - Neurotoxin

KW - Progenitor toxin

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SN - 0385-5600

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JO - Microbiology and Immunology

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Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike

Abstract

Keywords

ASJC Scopus subject areas

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