Similarity in Nucleotide Sequence of the Gene Encoding Nontoxic Component of Botulinum Toxin Produced by Toxigenic Clostridium butyricum Strain BL6340 and Clostridium botulinum Type E Strain Mashike

Nobuhiro Fujii, Kouichi Kimura, Noriko Yokosawa, Keiji Oguma, Teruo Yashiki, Touru Ohyama, T. Takeshi, Emiko Isogai, Hiroshi Isogai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb Hindlll fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C.butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.

Original languageEnglish
Pages (from-to)774-777
Number of pages4
JournalMICROBIOLOGY and IMMUNOLOGY
Volume37
Issue number5
DOIs
Publication statusPublished - 1993

Keywords

  • Botulinum toxin
  • Clostridium butyricum
  • Gene cloning
  • Neurotoxin
  • Progenitor toxin

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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