Silver diamine fluoride (SDF) used in childhood caries management has potent antifungal activity against oral Candida species

Kausar Sadia Fakhruddin, Hiroshi Egusa, Hien Chi Ngo, Chamila Panduwawala, Siripen Pesee, Thenmozhi Venkatachalam, Lakshman Perera Samaranayake

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC. Here we evaluate the antifungal effect of silver diamine fluoride (SDF) against 35-clinical yeast isolates (Ten-each of C. albicans, C. krusei, C. tropicalis and five C. glabrata strains) from dentinal caries-lesions from S-ECC. Results: Disc-diffusion and time-kill assays as well as MIC50 and MIC90 evaluations against therapeutic concentrations confirmed the broad-spectrum anti-candidal potency of SDF. Ultrastructural images revealed morphologic aberrations of yeast-cell walls on exposure to SDF. All C. krusei and C. glabrata isolates were significantly more sensitive to SDF, relative to the standard antifungal fluconazole. Further, SDF appears to effectively abrogate filamentation of C. albicans even at very low concentrations. Conclusions: Our data, for the first time, elucidate the antifungal potency of SDF, in addition to its known antibacterial activity, in the management of S-ECC.

Original languageEnglish
Article number95
JournalBMC Microbiology
Volume20
Issue number1
DOIs
Publication statusPublished - 2020 Apr 15

Keywords

  • Antifungal efficacy
  • Candida species
  • Dentin caries
  • Severe early childhood caries (S ECC)
  • Silver diamine fluoride

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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