Significance of xanthine oxidase in the production of intracellular oxygen radicals in an in-vitro hypoxia-reoxygenation model

Tetsuya Kakita, Masanori Suzuki, Heigo Takeuchi, Michiaki Unno, Seiki Matsuno

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Background/Purpose. The peroxidation of membranous phospholipids induced by ischemia reperfusion was inhibited in Cu/Zn superoxide dismutase (SOD) overexpressing mice, suggesting a detrimental role for intracellular reactive oxygen species (ROS) in reoxygenated cell injury. To ascertain the in vitro relevance of this hypothesis, the present study examined the participation of intracellular ROS in reoxygenation injury. Methods. This examination was done in two experimental models: Cu/Zn-SOD transgenic (Tg) mice that underwent hypoxia-reoxygenation in vitro and normal mice pretreated with a specific inhibitor of xanthine oxidase, BOF-4272, followed by in vitro hypoxia-reoxygenation. Results. The release of aspartate aminotransferase (AST) and the peroxidation of phospholipids were both ameliorated in hepatocytes from the Tg mice compared with findings in hepatocytes from normal mice. Similar findings were seen in the BOF-4272-pretreated cells, in which there was a decrease in AST and phospholipid peroxides. Conclusions. These results support the pivotal role of intracellular ROS generated by xanthine oxidase in reoxygenated cell injury, and suggest the viability of using an intracellular antioxidative therapy for reperfusion injury of the liver.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalJournal of Hepato-Biliary-Pancreatic Surgery
Volume9
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • Hepatic ischemia-reperfusion injury
  • Lipoperoxidation
  • Superoxide
  • Superoxide dismutase
  • Xanthine oxidase

ASJC Scopus subject areas

  • Surgery
  • Hepatology

Fingerprint Dive into the research topics of 'Significance of xanthine oxidase in the production of intracellular oxygen radicals in an in-vitro hypoxia-reoxygenation model'. Together they form a unique fingerprint.

Cite this