Signal-off and signal-on design for a label-free aptasensor based on target-induced self-assembly and abasic-site-binding ligands

Zhiai Xu, Yusuke Sato, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Getting an "A": A label-free adenosine aptasensor has been designed based on adenosine-induced selfassembly of aptamer fragments. One aptamer fragment contains an abasic (AP) site, and the AP-site-binding ligands (*) are used as fluorescence probes for signal-off and signal-on responses. The aptasensor can detect adenosine with high selectivity over the other three nucleotides.

Original languageEnglish
Pages (from-to)10375-10378
Number of pages4
JournalChemistry - A European Journal
Volume15
Issue number40
DOIs
Publication statusPublished - 2009 Oct 12

Keywords

  • Abasic sites
  • Aptamers
  • Fluorescent probes
  • Nucleobases
  • Self-assembly

ASJC Scopus subject areas

  • Chemistry(all)

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