Signal change of surface acoustic wave (SAW) caused by H2O 2 damage to SV40-T2 cells cultivated on SH-SAW sensor

Hideki Otori, Takumi Higashiyama, Atsushi Uehara, Miho Kainuma, Yukako Kudo, Toru Kamimura, Tasuku Kon, Katsumi Mochitate, Hideaki Kikuchi, Yasubumi Furuya

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


We developed a cellular biosensing system based on the direct assembly of rat- or human-cultured cells onto a shear horizontal-surface acoustic wave (SH-SAW) device. This novel design enables the sensitive monitoring of changes in physical cell-characteristics, such as viscosity or density inside the cells. In our system, the change of phase of SAW and insertion loss was observed to be time-dependent when the rat lung epithelial cells were treated with 1.0mM H2O2. Immunofluorescence microscopy showed the disappearance of the zonula occludens protein from the cell-cell contact region. Furthermore, the transepithelial electrical resistance of the cells gradually decreased owing to this treatment. These results suggest the correlation between the change of SH-SAW phase, the insertion loss of the SH-SAW parameter, and the destruction of the tight junction of the cells on SH-SAW devices. Crown

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalSensors and Actuators, A: Physical
Publication statusPublished - 2013
Externally publishedYes


  • Biosensor
  • Epithelial cell
  • Hydrogen peroxide
  • Shear horizontal-surface acoustic wave
  • Smart sensor
  • Tight junction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering


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