Three sialyl oligosaccharide fractions were separated from ovine colostrum by gel filtration, anion exchange chromatography and normal-phase HPLC. They were characterized by 1H-NMR spectrometry as follows: Neu5Acα2→3Galβ1→4Glc, Neu5Gcα2→6Galβ1→4Glc and three forms of Neu5Gcα2→3Galβ1→4Glc, namely Neu5Gcα2→3Galβ1→4Glc itself, its lactone derivative between the carboxyl group of Neu5Gc and Gal OH-2 and another lactone derivative between the carboxyl group and Gal OH-4. In this study, Neu5Gc-lactose lactones, in their free form, have been isolated for the first time from any natural sources including milk or colostrum. Copyright (C) 1998 Elsevier Science B.V.
ASJC Scopus subject areas
- Molecular Biology