Short communication: Characterization of DRB3 alleles in the MHC of Japanese Shorthorn cattle by polymerase chain reaction-sequence-based typing

S. Takeshima, Y. Nakai, M. Ohta, Y. Aida

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

A study was made of exon 2 of the bovine leukocyte antigen BoLA-DRB3 gene of 176 Japanese Shorthorn cattle at six farms in Japan using polymerase chain reaction-sequence-based typing (PCR-SBT). An initial round of PCR using conserved locus-specific primers, a second round of PCR using a locus-specific primer, and at least one sequence-specific primer (SSP), followed by direct sequencing of products of PCR with SSP were conducted. Twenty-one BoLA-DRB3 alleles were identified with frequencies ranging from 0.3 to 19.6% in 176 individuals, and two of these alleles were new alleles that have not been reported previously. The three most frequently observed alleles (DRBS*1201, *0301, and *0801) accounted for 43.8% of the alleles in the population of these herds. Next, we tested the products of amplification by PCR of BoLA-DRB3 exon 2 with RsaI, BstYI, and HaeIII, and identified 18 previously described PCR-restriction fragment length polymorphism (RFLP) types. The PCR-RFLP types reflected the results of PCR-SBT exactly. Our results indicate that exon 2 of the BoLA-DRB3 gene is highly polymorphic in Japanese Shorthorn cattle.

Original languageEnglish
Pages (from-to)1630-1632
Number of pages3
JournalJournal of Dairy Science
Volume85
Issue number6
DOIs
Publication statusPublished - 2002 Jun

Keywords

  • BoLA-DRB3 exon 2
  • Genetic polymorphism
  • Japanese Shorthorn cattle
  • PCR-sequence-based typing

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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