Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth

Tadayuki Shimada, Michinori Toriyama, Kaori Uemura, Hiroyuki Kamiguchi, Tadao Sugiura, Naoki Watanabe, Naoyuki Inagaki

    Research output: Contribution to journalArticlepeer-review

    82 Citations (Scopus)

    Abstract

    Actin polymerizes near the leading edge of nerve growth cones, and actin filaments show retrograde movement in filopodia and lamellipodia. Linkage between actin filament retrograde flow and cell adhesion molecules (CAMs) in growth cones is thought to be one of the mechanisms for axon outgrowth and guidance. However, the molecular basis for this linkage remains elusive. Here, we show that shootin1 interacts with both actin filament retrograde flow and L1-CAM in axonal growth cones of cultured rat hippocampal neurons, thereby mediating the linkage between them. Impairing this linkage, either by shootin1 RNA interference or disturbing the interaction between shootin1 and actin filament flow, inhibited L1-dependent axon outgrowth, whereas enhancing the linkage by shootin1 overexpression promoted neurite outgrowth. These results strengthen the actin flow-CAM linkage model ("clutch" model) for axon outgrowth and suggest that shootin1 is a key molecule involved in this mechanism.

    Original languageEnglish
    Pages (from-to)817-829
    Number of pages13
    JournalJournal of Cell Biology
    Volume181
    Issue number5
    DOIs
    Publication statusPublished - 2008 Jun 2

    ASJC Scopus subject areas

    • Cell Biology

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