Shifting the linear range in electrospray ionization by in-source collision-induced dissociation

Hideaki Ishii, Hiroaki Yamaguchi, Nariyasu Mano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The goal of this study was to demonstrate the utility of in-source collision-induced dissociation for shifting the linear range in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). The linear ranges for uracil, deoxyuridine, and uridine were shifted from 0.3-300, 1-100, and 10-1000 ng/mL to 10-1000, 30-3000, and 100-10000 ng/mL, respectively, by changing the declustering potential controlling in-source collision-induced dissociation. This technique should be considered for control of linear range in simultaneous quantitative measurements of extremely different amounts of compounds, drugs, and metabolites using LC/ESI-MS/MS.

Original languageEnglish
Pages (from-to)356-359
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume64
Issue number4
DOIs
Publication statusPublished - 2016 Apr

Keywords

  • Electrospray ionization
  • In-source collision-induced dissociation
  • Linear range

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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