TY - JOUR
T1 - Shifting the linear range in electrospray ionization by in-source collision-induced dissociation
AU - Ishii, Hideaki
AU - Yamaguchi, Hiroaki
AU - Mano, Nariyasu
PY - 2016/4
Y1 - 2016/4
N2 - The goal of this study was to demonstrate the utility of in-source collision-induced dissociation for shifting the linear range in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). The linear ranges for uracil, deoxyuridine, and uridine were shifted from 0.3-300, 1-100, and 10-1000 ng/mL to 10-1000, 30-3000, and 100-10000 ng/mL, respectively, by changing the declustering potential controlling in-source collision-induced dissociation. This technique should be considered for control of linear range in simultaneous quantitative measurements of extremely different amounts of compounds, drugs, and metabolites using LC/ESI-MS/MS.
AB - The goal of this study was to demonstrate the utility of in-source collision-induced dissociation for shifting the linear range in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). The linear ranges for uracil, deoxyuridine, and uridine were shifted from 0.3-300, 1-100, and 10-1000 ng/mL to 10-1000, 30-3000, and 100-10000 ng/mL, respectively, by changing the declustering potential controlling in-source collision-induced dissociation. This technique should be considered for control of linear range in simultaneous quantitative measurements of extremely different amounts of compounds, drugs, and metabolites using LC/ESI-MS/MS.
KW - Electrospray ionization
KW - In-source collision-induced dissociation
KW - Linear range
UR - http://www.scopus.com/inward/record.url?scp=84971620518&partnerID=8YFLogxK
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U2 - 10.1248/cpb.c15-00741
DO - 10.1248/cpb.c15-00741
M3 - Article
C2 - 27039832
AN - SCOPUS:84971620518
VL - 64
SP - 356
EP - 359
JO - Chemical and Pharmaceutical Bulletin
JF - Chemical and Pharmaceutical Bulletin
SN - 0009-2363
IS - 4
ER -