Abstract
The goal of this study was to demonstrate the utility of in-source collision-induced dissociation for shifting the linear range in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). The linear ranges for uracil, deoxyuridine, and uridine were shifted from 0.3-300, 1-100, and 10-1000 ng/mL to 10-1000, 30-3000, and 100-10000 ng/mL, respectively, by changing the declustering potential controlling in-source collision-induced dissociation. This technique should be considered for control of linear range in simultaneous quantitative measurements of extremely different amounts of compounds, drugs, and metabolites using LC/ESI-MS/MS.
| Original language | English |
|---|---|
| Pages (from-to) | 356-359 |
| Number of pages | 4 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 64 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2016 Apr |
Keywords
- Electrospray ionization
- In-source collision-induced dissociation
- Linear range
ASJC Scopus subject areas
- Chemistry(all)
- Drug Discovery
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Ishii, H., Yamaguchi, H., & Mano, N. (2016). Shifting the linear range in electrospray ionization by in-source collision-induced dissociation. Chemical and Pharmaceutical Bulletin, 64(4), 356-359. https://doi.org/10.1248/cpb.c15-00741