Shape anisotropy induces rotations in optically trapped red blood cells

Kapil Bambardekar, Jayashree A. Dharmadhikari, Aditya K. Dharmadhikari, Toshihoro Yamada, Tsuyoshi Kato, Hirohiko Kono, Yuichi Fujimura, Shobhona Sharma, Deepak Mathur

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A combined experimental and theoretical study is carried out to probe the rotational behavior of red blood cells (RBCs) in a single beam optical trap. We induce shape changes in RBCs by altering the properties of the suspension medium in which live cells float. We find that certain shape anisotropies result in the rotation of optically trapped cells. Indeed, even normal (healthy) RBCs can be made to rotate using linearly polarized trapping light by altering the osmotic stress the cells are subjected to. Hyperosmotic stress is found to induce shape anisotropies. We also probe the effect of the medium's viscosity on cell rotation. The observed rotations are modeled using a Langevin-type equation of motion that takes into account frictional forces that are generated as RBCs rotate in the medium. We observe good correlation between our measured data and calculated results.

Original languageEnglish
Article number041504
JournalJournal of Biomedical Optics
Volume15
Issue number4
DOIs
Publication statusPublished - 2010 Jul 1

Keywords

  • Cell elasticity
  • Cell rotation
  • Erythrocytes
  • Optical trap
  • Red blood cells

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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  • Cite this

    Bambardekar, K., Dharmadhikari, J. A., Dharmadhikari, A. K., Yamada, T., Kato, T., Kono, H., Fujimura, Y., Sharma, S., & Mathur, D. (2010). Shape anisotropy induces rotations in optically trapped red blood cells. Journal of Biomedical Optics, 15(4), [041504]. https://doi.org/10.1117/1.3430732