Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1

Mayumi Tabuchi, Kenjiro Sugiyama, Keiki Ishiyama, Eri Inoue, Tadashi Sato, Hideki Takahashi, Tomoyuki Yamaya

Research output: Contribution to journalArticlepeer-review

188 Citations (Scopus)

Abstract

Rice (Oryza sativa L.) plants possess three homologous but distinct genes for cytosolic glutamine synthetase (GS1): these are OsGS1;1, OsGS1;2, and OsGS1;3. OsGS1;1 was expressed in all organs tested with higher expression in leaf blades, while OsGS1;2, and OsGS1;3 were expressed mainly in roots and spikelets, respectively. We characterized knockout mutants caused by insertion of endogenous retrotransposon Tos17 into the exon-8 (lines ND8037 and ND9801) or the exon-10 (line NC2327) of OsGS1;7. Mendelian segregation occurred in each progeny. Homozygously inserted mutants showed severe retardation in growth rate and grain filling when grown at normal nitrogen concentrations. Abnormal mRNA for GS1;1 was transcribed, and the GS1 protein and its activity in the leaf blades were barely detectable in these mutants. The glutamine pool in the roots and leaf blades of the mutants was lower than that of the wild type. Re-introduction of OsGS1;1 cDNA under the control of its own promoter into the mutants successfully complemented these phenotypes. Progeny where Tos17 was heterozygously inserted or deleted during segregation showed normal phenotypes. The results indicate that GS1;1 is important for normal growth and grain filling in rice; GS1;2 and GS1;3 were not able to compensate for GS1;1 function.

Original languageEnglish
Pages (from-to)641-651
Number of pages11
JournalPlant Journal
Volume42
Issue number5
DOIs
Publication statusPublished - 2005 Jun

Keywords

  • Cytosolic glutamine synthetase
  • Knockout mutant
  • Nitrogen remobilization
  • Retrotransposon Tos17
  • Rice

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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