Severe chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gene and two host recessive genes.

H. Takahashi, Y. Ehara

Research output: Contribution to journalArticle

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Abstract

To investigate the genetic determinants of severe chlorotic spot symptoms in cucumber mosaic virus (CMV[Y])-inoculated tobacco (Nicotiana tabacum 'Ky57') leaves, symptom expression was compared among tobaccos (N. tabacum 'Ky57', N. tabacum 'Xanthi nc', and their hybrids) inoculated with CMV(Y), CMV(O), or pseudorecombinants of both strains of CMV. Xanthi nc showed very mild chlorotic spot symptoms in CMV(Y)-inoculated leaves. Both CMV(O)-inoculated Ky57 and Xanthi nc leaves showed no symptoms. In pseudorecombinant-inoculated Ky57 leaves, severe symptoms were determined by chimeric CMV(O) and CMV(Y) RNA3 transcripts, which contained most of the coat protein gene of CMV(Y). Genetic analysis of severe symptom formation in CMV(Y) or pseudorecombinant-inoculated tobaccos indicated that two recessive nuclear-coded host genes determined severe symptom formation in CMV(Y)-inoculated Ky57. The two recessive host genetic loci completely cosegregated with the two recessive burley loci (yb1 and yb2 genes) of Ky57, whose character is an apparent reduction in chlorophyll of the stems. When several cultivars of N. tabacum were inoculated with CMV(Y), all burley tobaccos clearly showed severe symptoms, but most other cultivars showed very mild symptoms. These results suggest that severe chlorotic spot symptom formation in CMV(Y)-inoculated Ky57 is induced by a combination of the virus coat protein gene and two recessive host genes that are closely linked to or identical to yb genes.

Original languageEnglish
Pages (from-to)182-189
Number of pages8
JournalMolecular plant-microbe interactions : MPMI
Volume6
Issue number2
DOIs
Publication statusPublished - 1993 Jan 1

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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