Serum Anti-p53 Antibody Can Serve as a Predictive Marker for Histological Grade of Intraductal Papillary Mucinous Neoplasms of the Pancreas

Tatsuo Hata, Masamichi Mizuma, Fuyuhiko Motoi, Masahiro Iseki, Yuko Omori, Hiroki Hayashi, Kei Nakagawa, Takanori Morikawa, Takashi Kamei, Takeshi Naitoh, Toru Furukawa, Michiaki Unno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective The aim of the study was to clarify the diagnostic impact of measuring serum anti-p53 antibody (S-p53Ab) in predicting the histological grades of intraductal papillary mucinous neoplasms (IPMNs) of the pancreas. Methods We compared the measured values and positive prevalence of S-p53Ab across the different histological grades of 111 resected IPMN cases. We also evaluated the TP53 alterations using immunohistochemistry and next-generation sequencing. Results Serum anti-p53 antibody were detected in 6 of 111 cases, all of their histological grades were high-grade dysplasia (HGD) and invasive carcinoma (INV). Positive prevalence of S-p53Ab was higher in cases with INV (4/35 cases, 11.4%) than those with HGD (2/38 cases, 5.3%), whereas S-p53Abs were undetectable in cases with low-grade dysplasia. Measured S-p53Ab values were not correlated with either carcinoembryonic antigen (CEA) or carbohydrate antigen 19-9 (CA 19-9). In 4 of 6 S-p53Ab-positive cases, the TP53 alterations-somatic pathogenic mutations or aberrant immunoreactivity-were identified in their IPMN lesions. A combination assay of S-p53Ab, CEA, and CA 19-9 revealed a 38.4% sensitivity and 81.6% specificity for predicting HGD/INV. Conclusions Serum anti-p53 antibody can serve as a surrogate marker for TP53 alterations and help predict the presence of HGD/INV in cases with IPMN, in combination with CEA and CA 19-9.

Original languageEnglish
Pages (from-to)768-773
Number of pages6
JournalPancreas
Volume49
Issue number6
DOIs
Publication statusPublished - 2020 Jul 1

Keywords

  • TP53
  • biomarker
  • grade of dysplasia
  • intraductal papillary mucinous neoplasms of the pancreas
  • next-generation sequencing
  • serum anti-p53 antibody

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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