Seroprevalence of chronic hepatitis B, as determined from dried blood spots, among children and their mothers in central Lao People's Democratic Republic: A multistage, stratified cluster sampling survey

Kenichi Komada, Masaya Sugiyama, Phengta Vongphrachanh, Anonh Xeuatvongsa, Bouaphan Khamphaphongphane, Tomomi Kitamura, Tomoko Kiyohara, Takaji Wakita, Hitoshi Oshitani, Masahiko Hachiya

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: There is limited information regarding the prevalence of hepatitis B in Lao PDR, where the hepatitis disease burden is substantial. Thus, reliable seroprevalence data is needed for the disease, based on probability sampling. Methods: A stratified, multistage, cluster sampling survey of hepatitis B surface antigen (HBsAg) positivity among children aged 5-9 years and their mothers aged 15-45 years was conducted. Participants were selected randomly from the central region of Lao PDR via probability-proportional-to-size sampling. Blood samples were collected onto filter paper and subsequently analyzed using a chemiluminescent microparticle immunoassay. Results: A total of 911 mother-and-child pairs were collected; the seroprevalence of HBsAg was estimated to be 2.1% (95% confidence interval 0.8-3.4%) among children and 4.1% (95% confidence interval 2.6-5.5%) in their mothers after taking into account the sampling design and the weight of each sample. The children's HBsAg positivity was positively associated with maternal infection and being born in a non-health facility, while the maternal infection status was not associated with any background characteristic. Conclusions: Lao PDR has a relatively lower HBsAg prevalence in the general population compared to surrounding countries. To ensure comparability to other countries and to future data, rapid field tests are recommended for a nationwide prevalence survey.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalInternational Journal of Infectious Diseases
Volume36
DOIs
Publication statusPublished - 2015 Jul 1

Keywords

  • Chemiluminescent microparticle immunoassay
  • Dried blood spots
  • Hepatitis B
  • Probability sampling
  • Seroprevalence
  • Southeast Asia

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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