Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect

Motohiro Morioka; Kohji Fukunaga; Takayuki Kawano; Shu Hasegawa; Kohjiro Korematsu; Yutaka Kai; Jun ichiro Hamada; Eishichi Miyamoto; Yukitaka Ushio

doi:10.1006/bbrc.1998.9783

Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect

Motohiro Morioka, Kohji Fukunaga, Takayuki Kawano, Shu Hasegawa, Kohjiro Korematsu, Yutaka Kai, Jun ichiro Hamada, Eishichi Miyamoto, Yukitaka Ushio

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Orthovanadate is known to be an inhibitor of protein tyrosine phosphatases. However, we found that it inhibited calcineurin which has the activity of a serine/threonine protein phosphatase, using casein phosphorylated by cyclic AMP-dependent protein kinase as a substrate. Orthovanadate inhibits the Mn²⁺-activated activity of purified calcineurin to 20%; this is not the case without Mn²⁺. Furthermore, 10 mM dithiothreitol (DTT) reversed the inhibitory effects of orthovanadate. Orthovanadate showed the same inhibitory effect for calcineurin activity in homogenates as for the purified enzyme; the inhibitory effect was reversed by DTT. These results indicate that orthovanadate inhibits not only protein tyrosine phosphatases as reported, but also serine/threonine phosphatase activity of calcineurin.

Original language	English
Pages (from-to)	342-345
Number of pages	4
Journal	Biochemical and biophysical research communications
Volume	253
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1998.9783
Publication status	Published - 1998 Dec 18
Externally published	Yes

Keywords

Calcineurin
Sodium orthovanadate

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1998.9783

Fingerprint Dive into the research topics of 'Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect'. Together they form a unique fingerprint.

Cite this

Morioka, M., Fukunaga, K., Kawano, T., Hasegawa, S., Korematsu, K., Kai, Y., Hamada, J. I., Miyamoto, E., & Ushio, Y. (1998). Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect. Biochemical and biophysical research communications, 253(2), 342-345. https://doi.org/10.1006/bbrc.1998.9783

Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect. / Morioka, Motohiro; Fukunaga, Kohji; Kawano, Takayuki; Hasegawa, Shu; Korematsu, Kohjiro; Kai, Yutaka; Hamada, Jun ichiro; Miyamoto, Eishichi; Ushio, Yukitaka.

In: Biochemical and biophysical research communications, Vol. 253, No. 2, 18.12.1998, p. 342-345.

Research output: Contribution to journal › Article › peer-review

Morioka, M, Fukunaga, K, Kawano, T, Hasegawa, S, Korematsu, K, Kai, Y, Hamada, JI, Miyamoto, E & Ushio, Y 1998, 'Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect', Biochemical and biophysical research communications, vol. 253, no. 2, pp. 342-345. https://doi.org/10.1006/bbrc.1998.9783

Morioka, Motohiro ; Fukunaga, Kohji ; Kawano, Takayuki ; Hasegawa, Shu ; Korematsu, Kohjiro ; Kai, Yutaka ; Hamada, Jun ichiro ; Miyamoto, Eishichi ; Ushio, Yukitaka. / Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect. In: Biochemical and biophysical research communications. 1998 ; Vol. 253, No. 2. pp. 342-345.

@article{a20124bdb66f4e68be76b2a768e90505,

title = "Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect",

abstract = "Orthovanadate is known to be an inhibitor of protein tyrosine phosphatases. However, we found that it inhibited calcineurin which has the activity of a serine/threonine protein phosphatase, using casein phosphorylated by cyclic AMP-dependent protein kinase as a substrate. Orthovanadate inhibits the Mn2+-activated activity of purified calcineurin to 20%; this is not the case without Mn2+. Furthermore, 10 mM dithiothreitol (DTT) reversed the inhibitory effects of orthovanadate. Orthovanadate showed the same inhibitory effect for calcineurin activity in homogenates as for the purified enzyme; the inhibitory effect was reversed by DTT. These results indicate that orthovanadate inhibits not only protein tyrosine phosphatases as reported, but also serine/threonine phosphatase activity of calcineurin.",

keywords = "Calcineurin, Sodium orthovanadate",

author = "Motohiro Morioka and Kohji Fukunaga and Takayuki Kawano and Shu Hasegawa and Kohjiro Korematsu and Yutaka Kai and Hamada, {Jun ichiro} and Eishichi Miyamoto and Yukitaka Ushio",

year = "1998",

month = dec,

day = "18",

doi = "10.1006/bbrc.1998.9783",

language = "English",

volume = "253",

pages = "342--345",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Serine/Threonine phosphatase activity of calcineurin is inhibited by sodium orthovanadate and dithiothreitol reverses the inhibitory effect

AU - Morioka, Motohiro

AU - Fukunaga, Kohji

AU - Kawano, Takayuki

AU - Hasegawa, Shu

AU - Korematsu, Kohjiro

AU - Kai, Yutaka

AU - Hamada, Jun ichiro

AU - Miyamoto, Eishichi

AU - Ushio, Yukitaka

PY - 1998/12/18

Y1 - 1998/12/18

N2 - Orthovanadate is known to be an inhibitor of protein tyrosine phosphatases. However, we found that it inhibited calcineurin which has the activity of a serine/threonine protein phosphatase, using casein phosphorylated by cyclic AMP-dependent protein kinase as a substrate. Orthovanadate inhibits the Mn2+-activated activity of purified calcineurin to 20%; this is not the case without Mn2+. Furthermore, 10 mM dithiothreitol (DTT) reversed the inhibitory effects of orthovanadate. Orthovanadate showed the same inhibitory effect for calcineurin activity in homogenates as for the purified enzyme; the inhibitory effect was reversed by DTT. These results indicate that orthovanadate inhibits not only protein tyrosine phosphatases as reported, but also serine/threonine phosphatase activity of calcineurin.

AB - Orthovanadate is known to be an inhibitor of protein tyrosine phosphatases. However, we found that it inhibited calcineurin which has the activity of a serine/threonine protein phosphatase, using casein phosphorylated by cyclic AMP-dependent protein kinase as a substrate. Orthovanadate inhibits the Mn2+-activated activity of purified calcineurin to 20%; this is not the case without Mn2+. Furthermore, 10 mM dithiothreitol (DTT) reversed the inhibitory effects of orthovanadate. Orthovanadate showed the same inhibitory effect for calcineurin activity in homogenates as for the purified enzyme; the inhibitory effect was reversed by DTT. These results indicate that orthovanadate inhibits not only protein tyrosine phosphatases as reported, but also serine/threonine phosphatase activity of calcineurin.

KW - Calcineurin

KW - Sodium orthovanadate

UR - http://www.scopus.com/inward/record.url?scp=0032545336&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032545336&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.9783

DO - 10.1006/bbrc.1998.9783

M3 - Article

C2 - 9878539

AN - SCOPUS:0032545336

VL - 253

SP - 342

EP - 345

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -