Serine protease inhibitor cvSI-1 potential role in the eastern oyster host defense against the protozoan parasite Perkinsus marinus

Jerome F. La Peyre, Qing Gang Xue, Naoki Itoh, Yanli Li, Richard K. Cooper

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

The serine protease inhibitor cvSI-1, purified from plasma of eastern oysters, inhibited the proliferation of the protozoan parasite Perkinsus marinus in vitro. In situ hybridization located cvSI-1 gene expression in basophil cells of the digestive tubules and cvSI-1 expression measured by real-time quantitative reverse transcriptase polymerase chain reaction was several hundred folds greater in digestive glands than in other organs examined or circulating hemocytes. cvSI-1 gene expression was also significantly greater in winter than in summer. Finally, cvSI-1 gene expression and plasma protease inhibitory activity in oysters selected for increased resistance to P. marinus were significantly greater than in unselected oysters. These findings support the hypothesis that cvSI-1 plays a role in eastern oyster host defense against P. marinus possibly through inhibition of parasite proliferation.

Original languageEnglish
Pages (from-to)84-92
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume34
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Keywords

  • Bivalve mollusks
  • Crassostrea virginica
  • Dermo
  • Disease resistance
  • Genetic selection
  • In situ hybridization
  • Innate immunity
  • Plasma protein
  • Quantitative real-time RT-PCR

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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