Abstract
Oxygen consumption rate (OCR) of mouse embryoid body (EB) was measured repeatedly in glucose-depleted medium or PBS solution. Non-invasive nature of the scanning electrochemical microscopy allowed sequential OCR measurement of the identical EB sample for more than 24 h. We found that the OCR value for the undifferentiated EB (cultured for 2 days) reached almost zero after 12 h exposure in PBS. On the contrary, the OCR value for the differentiated EB (cultured for 8 days) indicated 40 to 28% normal to the OCR at 0 h-incubation after 24 h exposure in PBS-based solution. It is suggested that differentiated cells indicated a stronger adaptation to glucose deprivation comparing to the undifferentiated cells.
Original language | English |
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Pages (from-to) | 302-304 |
Number of pages | 3 |
Journal | Electrochemistry |
Volume | 84 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2016 May |
Keywords
- Embryonic stem cell
- Oxygen consumption
- Scanning electrochemical microscopy
ASJC Scopus subject areas
- Electrochemistry