Sequential monitoring of oxygen consumption rate of mouse embryoid bodies in glucose-depleted solution

Hitoshi Shiku, Nana Aoki, Toshiharu Arai, Yuanshu Zhou, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Oxygen consumption rate (OCR) of mouse embryoid body (EB) was measured repeatedly in glucose-depleted medium or PBS solution. Non-invasive nature of the scanning electrochemical microscopy allowed sequential OCR measurement of the identical EB sample for more than 24 h. We found that the OCR value for the undifferentiated EB (cultured for 2 days) reached almost zero after 12 h exposure in PBS. On the contrary, the OCR value for the differentiated EB (cultured for 8 days) indicated 40 to 28% normal to the OCR at 0 h-incubation after 24 h exposure in PBS-based solution. It is suggested that differentiated cells indicated a stronger adaptation to glucose deprivation comparing to the undifferentiated cells.

Original languageEnglish
Pages (from-to)302-304
Number of pages3
JournalElectrochemistry
Volume84
Issue number5
DOIs
Publication statusPublished - 2016 May

Keywords

  • Embryonic stem cell
  • Oxygen consumption
  • Scanning electrochemical microscopy

ASJC Scopus subject areas

  • Electrochemistry

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