TY - GEN
T1 - Sequential assembly of the functional material micropatterns on the hydrogel sheet for constructing skeletal muscle cell-based assay system
AU - Nagamine, Kuniaki
AU - Otani, Shingo
AU - Ito, Syuntaro
AU - Kaji, Hirokazu
AU - Kanzaki, Makoto
AU - Nishizawa, Matsuhiko
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The functional material micropatterns-assembled hydrogel sheet has been prepared by means of sequential transfer technique to construct the skeletal muscle cell-based assay system. We demonstrated to construct micropatterned synaptic connection between motor neurons-skeletal muscle cells using this technique. Besides, assembly of the non-biological functional materials has also advantage to confer additional functions to a hydrogel assay system. The fluorescent microbeads were assembled onto a hydrogel as sensing elements of contractile force and metabolic activity of skeletal muscle cells. These flexible sensor sheets would be useful to study the relationship between exercise and metabolic activity of muscle in type 2 diabetes.
AB - The functional material micropatterns-assembled hydrogel sheet has been prepared by means of sequential transfer technique to construct the skeletal muscle cell-based assay system. We demonstrated to construct micropatterned synaptic connection between motor neurons-skeletal muscle cells using this technique. Besides, assembly of the non-biological functional materials has also advantage to confer additional functions to a hydrogel assay system. The fluorescent microbeads were assembled onto a hydrogel as sensing elements of contractile force and metabolic activity of skeletal muscle cells. These flexible sensor sheets would be useful to study the relationship between exercise and metabolic activity of muscle in type 2 diabetes.
KW - Assembly
KW - Bioassay
KW - Hydrogel
KW - Skeletal muscle cell
UR - http://www.scopus.com/inward/record.url?scp=84901810703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901810703&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901810703
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1054
EP - 1056
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -
