TY - JOUR
T1 - Sequencing and characterization of the xyl operon of a gram-positive bacterium, Tetragenococcus halophila
AU - Takeda, Yasuo
AU - Takase, Kazuma
AU - Yamato, Ichiro
AU - Abe, Keietsu
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1998/7
Y1 - 1998/7
N2 - The xyl operon of a gram-positive bacterium, Tetragenococcus halophila (previously called Pediococcus halophilus), was cloned and sequenced. The DNA was about 7.7 kb long and contained genes for a ribose binding protein and part of a ribose transporter, xylR (a putative regulatory gene), and the xyl operon, along with its regulatory region and transcription termination signal, in this order. The DNA was AT rich, the GC content being 35.8%, consistent with the GC content of this gram-positive bacterium. The xyl operon consisted of three genes, xylA, encoding a xylose isomerase, xylB, encoding a xylulose kinase, and xylE, encoding a xylose transporter, with predicted molecular weights of 49,400, 56,400, and 51,600, respectively. The deduced amino acid sequences of the XyIR, XyIA, XyIB, and XyIE proteins were similar to those of the corresponding proteins in other gram-positive and - negative bacteria, the similarities being 37 to 64%. Each polypeptide of XyIB and XyIE was expressed functionally in Escherichia coli. XyIE transported o- xylose in a sodium ion-dependent manner, suggesting that it is the first described xylose/Na+ symporter. The XyIR protein contained a consensus sequence for binding catabolites of glucose, such as glucose-6-phosphate, which has been discovered in glucose and fructose kinases in bacteria. Correspondingly, the regulatory region of this operon contained a putative binding site of XyIR with a palindromic structure. Furthermore, it contained a consensus sequence, CRE (catabolite-responsive element), for binding CcpA (catabolite control protein A). We speculate that the transcriptional regulation of this operon resembles the regulation of catabolite-repressible operons such as the amy, lev, xyl, and gnt operons in various gram-positive bacteria. We discuss the significance of the regulation of gene expression of this operon in T. halophila.
AB - The xyl operon of a gram-positive bacterium, Tetragenococcus halophila (previously called Pediococcus halophilus), was cloned and sequenced. The DNA was about 7.7 kb long and contained genes for a ribose binding protein and part of a ribose transporter, xylR (a putative regulatory gene), and the xyl operon, along with its regulatory region and transcription termination signal, in this order. The DNA was AT rich, the GC content being 35.8%, consistent with the GC content of this gram-positive bacterium. The xyl operon consisted of three genes, xylA, encoding a xylose isomerase, xylB, encoding a xylulose kinase, and xylE, encoding a xylose transporter, with predicted molecular weights of 49,400, 56,400, and 51,600, respectively. The deduced amino acid sequences of the XyIR, XyIA, XyIB, and XyIE proteins were similar to those of the corresponding proteins in other gram-positive and - negative bacteria, the similarities being 37 to 64%. Each polypeptide of XyIB and XyIE was expressed functionally in Escherichia coli. XyIE transported o- xylose in a sodium ion-dependent manner, suggesting that it is the first described xylose/Na+ symporter. The XyIR protein contained a consensus sequence for binding catabolites of glucose, such as glucose-6-phosphate, which has been discovered in glucose and fructose kinases in bacteria. Correspondingly, the regulatory region of this operon contained a putative binding site of XyIR with a palindromic structure. Furthermore, it contained a consensus sequence, CRE (catabolite-responsive element), for binding CcpA (catabolite control protein A). We speculate that the transcriptional regulation of this operon resembles the regulation of catabolite-repressible operons such as the amy, lev, xyl, and gnt operons in various gram-positive bacteria. We discuss the significance of the regulation of gene expression of this operon in T. halophila.
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U2 - 10.1128/aem.64.7.2513-2519.1998
DO - 10.1128/aem.64.7.2513-2519.1998
M3 - Article
C2 - 9647823
AN - SCOPUS:0031841222
VL - 64
SP - 2513
EP - 2519
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 7
ER -