Sequencing and characterization of the kinesin-related genes katB and katC of Arabidopsis thaliana

Hisayuki Mitsui, Kimihiko Nakatani, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki, Ken Nishikawa, Hideo Takahashi

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Complementary DNAs of two kinesin-related genes, katB and katC, were isolated from Arabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long α-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207-754)), which includes the carboxylterminal region of KatC, was expressed in Escherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.

Original languageEnglish
Pages (from-to)865-876
Number of pages12
JournalPlant Molecular Biology
Volume25
Issue number5
DOIs
Publication statusPublished - 1994 Aug
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • kat genes
  • kinesin-like proteins
  • microtubule-stimulated ATPase
  • nucleotide sequences

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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