Sequence determination of peptide by the combined use of fluoresceinisothiocyanate and phenylisothiocyanate

Koji Muramoto, Hiroshi Kawauchi, Katura Tuzimura

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    A fluorometric manual technique has been demonstrated for determining the amino acid sequence of a polypeptide, by the combined use of fluoresceinisothiocyanate (FITC) and phenylisothiocyanate (pITC). This method permits continuous assignments of 10 residues with 5.6 nmoles of myoglobin or 23 residues with 45 nmoles.

    Original languageEnglish
    Pages (from-to)1559-1563
    Number of pages5
    JournalAgricultural and Biological Chemistry
    Volume42
    Issue number8
    DOIs
    Publication statusPublished - 1978 Jan 1

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

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