Sequence comparisons among dispersed members of the Brassica S multigene family in an S9 genome

N. Kai, G. Suzuki, M. Watanabe, A. Isogai, K. Hinata

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Self-incompatibility (SI) systems prevent self-pollination and promote outbreeding. In Brassica, the SI genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are members of the S multigene family, which share the SLG-like domain (S domain), which encodes a putative receptor. We have cloned members of the S multigene family from the S9 haplotype of B. campestris (syn. rapa). In addition, eight distinct genomic regions harboring 10 SLG/SRK-like genes were characterized in the present study. Sequence analysis revealed two novel SRK-like genes, BcRK3 and BcRK6 (for B. campestris receptor kinases 3 and 6, respectively). Other genes that were characterized included SFR2 (for S gene family receptor 2), SLR2 (for S locus related gene 2), and a pseudogene. Based on phylogenetic analysis of the nucleotide sequences of the S domain regions, SLG and SRK appear to be distinct from other members of the S multigene family. Linkage analysis showed that most members of the S multigene family are dispersed in the Brassica genome, and that SLR1 (S locus related gene 1) is not linked to the SLR2 in B. campestris.

Original languageEnglish
Pages (from-to)526-534
Number of pages9
JournalMolecular and General Genetics
Volume265
Issue number3
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • Brassica campestris (syn. rapa)
  • Linkage analysis
  • Receptor-like kinase
  • S multigene family
  • Self-incompatibility

ASJC Scopus subject areas

  • Genetics

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