Sequence and structural diversity of the S locus genes from different lines with the same self-recognition specificities in Brassica oleracea

Makoto Kusaba, Masanori Matsushita, Keiichi Okazaki, Yoko Satta, Takeshi Nishio

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Self-incompatibility (SI) is a mechanism for preventing self- fertilization in flowering plants. In Brassica, it is controlled by a single multi-allelic locus, S, and it is believed that two highly polymorphic genes in the S locus, SLG and SRK, play central roles in self-recognition in stigmas. SRK is a putative receptor protein kinase, whose extracellular domain exhibits high similarity to SLG. We analyzed two pairs of lines showing cross-incompatibility (S2 and S(2-b); S13 and S(13-b)). In S2 and S(2-b), SRKs were more highly conserved than SLGs. This was also the case with S13 and S(13-b). This suggests that the SRKs of different lines must be conserved for the lines to have the same self-recognition specificity. In particular, SLG(2-b) showed only 88.5% identity to SLG2, which is comparable to that between the SLGs of different S haplotypes, while SRK(2-b) showed 97.3% identity to SRK2 in the S domain. These findings suggest that the SLGs in these S haplotypes are not important for self-recognition in SI.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalGenetics
Volume154
Issue number1
Publication statusPublished - 2000 Jan 1

ASJC Scopus subject areas

  • Genetics

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