Sequence analysis of the germ-line VH gene corresponding to a nephritogenic antibody in MRL/lpr lupus mice

Masao Ono, T. Yamamoto, M. Kyogoku, Masato Nose

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to investigate the genetic origin of nephritogenic antibodies in MRL/Mp-lpr/lpr (MRL/lpr) lupus mice, we isolated the germ-line heavy chain variable region (V(H)) gene corresponding to the nephritogenic antibody, B1, derived from an unmanipulated MRL/lpr mouse. Injection of this antibody into C.B-17/Icr-scid/scid mice resulted in the generation of wire loop-like glomerular lesions resembling those of lupus nephritis. Nucleotide sequences of this germ-line V(H) gene showed no replacement mutation in the V(H) region of the B1 antibody. Furthermore, this gene was identical to that found in the C3H/HeJ-lpr/lpr strain of mice. Our results suggest that germ-line V(H) genes can encode nephritogenic antibodies without somatic mutation, even in a mouse strain not prone to lupus.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalClinical and Experimental Immunology
Volume100
Issue number2
DOIs
Publication statusPublished - 1995

Keywords

  • Germ-line V(H) gene
  • Lupus nephritis
  • MRL/lpr mouse
  • Nephritogenic antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Sequence analysis of the germ-line VH gene corresponding to a nephritogenic antibody in MRL/lpr lupus mice'. Together they form a unique fingerprint.

  • Cite this