Separation selectivity of anionic metal complexes of N,N'- bis(hydroxybenzyl)ethylenediamine-N,N'-diacetic acid in ion and ion electrokinetic chromatography

O. V. Krokhin, H. Hoshino, O. A. Shpigun, T. Yotsuyanagi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The complexes of Fe(III), Co(III), Mn(III), Al(III), Cu(II), Ni(II), Cd(II) and Zn(II) with N,N'-bis(hydroxybenzyl)ethylenediamine-N,N'-diacetic acid (HBED) were separated by ion exchange in different modes: ion chromatography (IC) and ion electrokinetic chromatography (IEKC). In column IC these complexes were separated on an IonPac AS4a anion-exchange column (Dionex, USA). Parameters of the background electrolyte that were examined in IEKC mode include polymer, competing ion concentration and pH. The use of poly(diallyldimethylammonium chloride) (PDADMACl) as a modifier in IEKC provides separation selectivity only slightly different from that observed in IC on the IonPac AS4a column. Optimal separation conditions were found to be: 0.1 mM HBED, 50 mM PDADMAOH, 10 mM Na2B4O7, pH adjusted to 10 with acetic acid. The use of an aromatic ligand allowed a 10-fold decrease in detection limits of metal ions in comparison with previously studied EDTA. A separation efficiency up to 400 000 theoretical plates was demonstrated for IEKC. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalJournal of Chromatography A
Volume895
Issue number1-2
DOIs
Publication statusPublished - 2000 Oct 20

Keywords

  • Bis(hydroxybenzyl)ethylenediamine- N,N'-diacetic acid
  • Complexation
  • Electrokinetic chromatography
  • Ion electrokinetic chromatography
  • Metal chelates
  • Selectivity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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