Separation of proteins BY continuous isoelectric focusing

Shuang Ning Zheng, Toshikuni Yonemoto, Teiriki Tadaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An experimental investigation of continuous electrophoretic separation of bovine serum albumin from bovine hemoglobin was carried out. The separation behavior was analyzed with the model equations presented in our previous paper. We examined the effect of operating parameters such as applied voltage, residence time and the buffer added to feed solution including carrier ampholyte, on the basis of experimental results and theoretical predications, and showed that 1) an optimum applied voltage exists for the electrophoretic separation process of proteins, and 2) the buffer concentration greatly influences the pH distribution and the separation behavior of proteins.

Original languageEnglish
Pages (from-to)471-476
Number of pages6
JournalJOURNAL of CHEMICAL ENGINEERING of JAPAN
Volume24
Issue number4
DOIs
Publication statusPublished - 1991 Jan 1

Keywords

  • Albumin
  • Biochemical Engineering
  • Hemoglobin
  • Isoelectric Focusing
  • Protein
  • Separation

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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