Separation of oligonucleotides with single-base mutation by capillary electrophoresis using specific interaction of metal ion with nucleotide

Toru Takahashi, Takao Sakurai, Koichiro Hirata, Hitoshi Hoshino

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A unique tactic for the separation of single-base sequential isomers of oligomeric single-stranded DNA by a CE separation system employing the specific interaction of metal ion with nucleotide was demonstrated, enabling the separation of the mixture of a 12-mer oligonucleotide and its single-base mutants, as well as their positional isomers.

Original languageEnglish
Pages (from-to)1299-1301
Number of pages3
JournalAnalyst
Volume134
Issue number7
DOIs
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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