Separation and determination of glycolipids from edible plant sources by high-performance liquid chromatography and evaporative light-scattering detection

Tatsuya Sugawara, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

163 Citations (Scopus)

Abstract

Glycolipids from edible plant sources were accurately quantified by silica-based, normal-phase high-performance liquid chromatography using an evaporative light-scattering detector. Five major glycolipid classes (acylated steryl glucoside, steryl glucoside, ceramide monohexoside, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol) were separated and determined with a binary gradient system consisting of chloroform and methanol/water (95:5, vol/vol) without any interference from other lipid classes and pigments. The described method was applied to 48 edible plants available in Japan including cereals, legumes, vegetables, and fruits. Examined plant species contained glycolipids in wide concentration ranges, such as 5-645 mg/100 g tissue.

Original languageEnglish
Pages (from-to)1231-1237
Number of pages7
JournalLipids
Volume34
Issue number11
DOIs
Publication statusPublished - 1999 Jan 1

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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