TY - JOUR
T1 - Separation and characterization of ursodeoxycholate 7-n-acetylglu-cosaminides in human urine by high-performance liquid chromatography with fluorescence detection
AU - Niwa, Toshifumi
AU - Fujita, Kazuhiro
AU - Goto, Junichi
AU - Nambara, Toshio
N1 - Funding Information:
The authors express their sincere thanks to Dr. Naoaki Tanno (School of Medicine, Tohoku University) for providing the urine specimen. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1993/8/1
Y1 - 1993/8/1
N2 - The separation and characterization of unconjugated, glycine- and taurine-conjugated ursodeoxycholate 7-N-acetyl-glucosaminides in human urine without prior deconjugation have been carried out by high-performance liquid chromatography (HPLC) on a reversed phase column. The bile acid fraction was obtained from a urine specimen by the combined use of a Sep-Pakc18 cartridge for solid phase extraction and lipophilic gel, piperidinohydroxypropyl Sephadex LH-20 (PHP-LH-20), for ion-exchange chromatography. Bile acid N-acetyl-glucosaminides were derivatized quantitatively into the corresponding fluorescent esters through the inherent primary hydroxyl group by treatment with 9-anthroyl cyanide. The derivatives were separated into unconjugated, glycine- and taurine-conjugated fractions on PHP-LH-20. Subsequent resolution into individual N-acetylglucosaminides was attained.
AB - The separation and characterization of unconjugated, glycine- and taurine-conjugated ursodeoxycholate 7-N-acetyl-glucosaminides in human urine without prior deconjugation have been carried out by high-performance liquid chromatography (HPLC) on a reversed phase column. The bile acid fraction was obtained from a urine specimen by the combined use of a Sep-Pakc18 cartridge for solid phase extraction and lipophilic gel, piperidinohydroxypropyl Sephadex LH-20 (PHP-LH-20), for ion-exchange chromatography. Bile acid N-acetyl-glucosaminides were derivatized quantitatively into the corresponding fluorescent esters through the inherent primary hydroxyl group by treatment with 9-anthroyl cyanide. The derivatives were separated into unconjugated, glycine- and taurine-conjugated fractions on PHP-LH-20. Subsequent resolution into individual N-acetylglucosaminides was attained.
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U2 - 10.1080/10826079308019590
DO - 10.1080/10826079308019590
M3 - Article
AN - SCOPUS:0027334987
VL - 16
SP - 2531
EP - 2544
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 12
ER -