TY - JOUR
T1 - Sensitivity of genera Porphyromonas and Prevotella to the bactericidal action of C-terminal domain of human CAP18 and its analogues
AU - Isogai, Emiko
AU - Isogai, H.
AU - Matuo, K.
AU - Hirose, K.
AU - Kowashi, Y.
AU - Okumuara, K.
AU - Hirata, M.
PY - 2003/10
Y1 - 2003/10
N2 - This paper reports the effect of the synthesized 27-amino acid sequence in the C-terminal domain of human CAP18 (hCAP18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera Porophyromonas and Prevotella. The domain binds lipopolysaccharides (LPS) from Porophyromonas gingivalis and Porophyromonas circumdentaria as well as enterobacterial LPS. Two analogues of hCAP18, designated LL/CAP18 and FF/CAP18, were also tested to determine whether additional activity was obtained. The analogue peptides replaced with hydrophobic and cationic amino acid residues showed more potent bactericidal and LPS-binding activities than the original one.
AB - This paper reports the effect of the synthesized 27-amino acid sequence in the C-terminal domain of human CAP18 (hCAP18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera Porophyromonas and Prevotella. The domain binds lipopolysaccharides (LPS) from Porophyromonas gingivalis and Porophyromonas circumdentaria as well as enterobacterial LPS. Two analogues of hCAP18, designated LL/CAP18 and FF/CAP18, were also tested to determine whether additional activity was obtained. The analogue peptides replaced with hydrophobic and cationic amino acid residues showed more potent bactericidal and LPS-binding activities than the original one.
KW - Antimicrobial activity
KW - LPS-binding activity
KW - Lipopolysaccharide
KW - Porphyromonas
KW - hCAP18-derived peptides
UR - http://www.scopus.com/inward/record.url?scp=0041736471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041736471&partnerID=8YFLogxK
U2 - 10.1034/j.1399-302X.2003.00083.x
DO - 10.1034/j.1399-302X.2003.00083.x
M3 - Article
C2 - 12930528
AN - SCOPUS:0041736471
VL - 18
SP - 329
EP - 332
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
SN - 2041-1006
IS - 5
ER -