Sensitivity of genera Porphyromonas and Prevotella to the bactericidal action of C-terminal domain of human CAP18 and its analogues

Emiko Isogai; H. Isogai; K. Matuo; K. Hirose; Y. Kowashi; K. Okumuara; M. Hirata

doi:10.1034/j.1399-302X.2003.00083.x

Sensitivity of genera Porphyromonas and Prevotella to the bactericidal action of C-terminal domain of human CAP18 and its analogues

Emiko Isogai, H. Isogai, K. Matuo, K. Hirose, Y. Kowashi, K. Okumuara, M. Hirata

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

This paper reports the effect of the synthesized 27-amino acid sequence in the C-terminal domain of human CAP18 (hCAP18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera Porophyromonas and Prevotella. The domain binds lipopolysaccharides (LPS) from Porophyromonas gingivalis and Porophyromonas circumdentaria as well as enterobacterial LPS. Two analogues of hCAP18, designated LL/CAP18 and FF/CAP18, were also tested to determine whether additional activity was obtained. The analogue peptides replaced with hydrophobic and cationic amino acid residues showed more potent bactericidal and LPS-binding activities than the original one.

Original language	English
Pages (from-to)	329-332
Number of pages	4
Journal	Oral Microbiology and Immunology
Volume	18
Issue number	5
DOIs	https://doi.org/10.1034/j.1399-302X.2003.00083.x
Publication status	Published - 2003 Oct

Keywords

Antimicrobial activity
LPS-binding activity
Lipopolysaccharide
Porphyromonas
hCAP18-derived peptides

ASJC Scopus subject areas

Microbiology
Immunology
Dentistry(all)
Microbiology (medical)

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title = "Sensitivity of genera Porphyromonas and Prevotella to the bactericidal action of C-terminal domain of human CAP18 and its analogues",

abstract = "This paper reports the effect of the synthesized 27-amino acid sequence in the C-terminal domain of human CAP18 (hCAP18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera Porophyromonas and Prevotella. The domain binds lipopolysaccharides (LPS) from Porophyromonas gingivalis and Porophyromonas circumdentaria as well as enterobacterial LPS. Two analogues of hCAP18, designated LL/CAP18 and FF/CAP18, were also tested to determine whether additional activity was obtained. The analogue peptides replaced with hydrophobic and cationic amino acid residues showed more potent bactericidal and LPS-binding activities than the original one.",

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AU - Matuo, K.

AU - Hirose, K.

AU - Kowashi, Y.

AU - Okumuara, K.

AU - Hirata, M.

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